论文部分内容阅读
目的探讨5,10-亚甲基四氢叶酸还原酶(MTHFR)基因多态性与妊娠期高血压疾病的相关性。方法选取2013年6月至2014年6月收治的60例妊娠期高血压疾病患者作为试验组,另选择同时期无妊娠期高血压疾病的孕妇60例作为对照组,采用PCR技术对两组患者MTHFR C677T及A1298C基因进行检测。结果试验组MTHFR C677T C/T基因型频率明显高于对照组(χ2=4.03,P<0.05);试验组突变T等位基因频率显著高于对照组(χ2=4.26,P<0.05)。试验组与对照组MTHFR A1298C A/A、A/C、C/C三种基因型频率均无明显差异(P>0.05)。A1298C杂合子联合C667T杂合子基因型发生妊娠期高血压疾病的危险性无明显差异(P>0.05)。结论 MTHFR基因A1298C多态性与妊娠期高血压疾病的发病无明显相关性,而MTHFR基因C677T多态性可作为诱发妊娠期高血压疾病的危险因素,同时可将其作为评价妊娠期高血压疾病预后情况的一项指标,应引起临床工作者的重视。
Objective To investigate the relationship between 5,10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and gestational hypertension. Methods Sixty pregnant women with gestational hypertension who were admitted to our hospital from June 2013 to June 2014 were selected as the experimental group. Another 60 pregnant women with no pregnancy-induced hypertension were selected as the control group. MTHFR C677T and A1298C genes were detected. Results The frequency of MTHFR C677T C / T genotype in the experimental group was significantly higher than that in the control group (χ2 = 4.03, P <0.05). The frequency of the mutant T allele in the experimental group was significantly higher than that in the control group (χ2 = 4.26, P <0.05). The frequency of MTHFR A1298C A / A, A / C and C / C genotypes in experimental group and control group had no significant difference (P> 0.05). There was no significant difference in the risk of hypertensive disorder complicating pregnancy between A1298C heterozygote and C667T heterozygous genotype (P> 0.05). Conclusion There is no significant correlation between MTHFR gene A1298C polymorphism and the incidence of hypertensive disorder complicating pregnancy, while MTHFR gene C677T polymorphism can be used as a risk factor for hypertensive disorder induced by pregnancy, at the same time it can be used as a marker to evaluate gestational hypertension An indicator of the prognosis should arouse the attention of clinicians.