【摘 要】
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The Cyclophragma undans nucleopolyhedrovirus (CyunNPV),a potential pest control agent,was isolated from Cyclophragma undans (Lepidoptera:Lasiocampidae),an important forest pest.In the present study,we performed detailed genome analysis of CyunNPV and comp
【机 构】
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State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of V
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The Cyclophragma undans nucleopolyhedrovirus (CyunNPV),a potential pest control agent,was isolated from Cyclophragma undans (Lepidoptera:Lasiocampidae),an important forest pest.In the present study,we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group Ⅰ alphabaculovimses.Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45％.Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes,24 lepidopteran baculovirus conserved genes,nine Group Ⅰ Alphabaculovirus conserved genes,71 common genes,and five genes that are unique to CyunNPV.In addition,the genome contains 13 homologous repeated sequences (hrs).Phylogenetic analysis groups CyunNPV under a distinct branch within clade “a” of Group Ⅰ in the genus Alphabaculovirus.Unlike other members of Group Ⅰ,CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group Ⅰ viruses.Furthermore,the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene.The CyunNPV F-like protein contains two insertions of continuous polar amino acids,one at the conventional fusion peptide and a second insertion at the pretransmembrane domain.The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein.The above findings imply that CyunNPV is a distinct species under Group Ⅰ Alphabaculovirus.
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