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目的:观察维生素E琥珀酸酯(VES)对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。方法:将40只移植了H22肝癌瘤株的昆明种小鼠随机分成4组:模型对照组(仅腹腔注射芝麻油);VES低、中、高浓度组(分别予以VES 50、100、200 mg.kg-1),每组连续用药12 d。比较各组肿瘤重量、肿瘤生长抑制率、肿瘤细胞周期的变化、血清中TNF-α及IL-2的表达。结果:50 mg.kg-1VES组肿瘤重量为(1.98±0.56)g,与模型对照组比较,无肿瘤生长抑制作用(P>0.05);100、200 mg.kg-1VES组肿瘤重量分别为(1.15±0.32)g、(0.90±0.19)g,肿瘤生长抑制率分别为46.76%、58.33%,与模型对照组比较,差异均有统计学意义(P<0.05),且表现为浓度依赖关系;VES各组均使G0/G1期细胞比例增加,S期细胞比例降低;VES 100、200 mg.kg-1可显著增加小鼠血清中TNF-α及IL-2的表达(均P<0.05)。结论:VES在体内对小鼠H22肿瘤细胞的生长有明显的抑制作用,其作用机制可能与阻滞细胞周期于G0/G1期、上调血清中TNF-α及IL-2表达有关。
Objective: To observe the inhibitory effect of vitamin E succinate (VES) on H22 tumor-bearing mice and to explore its mechanism. Methods: Forty Kunming mice transplanted with H22 hepatocarcinoma were randomly divided into 4 groups: model control group (intraperitoneal injection of sesame oil only); VES low, medium and high concentration groups (VES 50, 100, 200 mg, respectively). kg-1), each group continuous medication for 12 d. The tumor weight, tumor growth inhibition rate, tumor cell cycle changes, serum TNF-α and IL-2 expression were compared. Results: The tumor weight in 50 mg · kg-1VES group was (1.98 ± 0.56) g, which showed no tumor growth inhibition compared with the model control group (P> 0.05). The tumor weights in 100 and 200 mg · kg-1VES groups were 1.15 ± 0.32) g and (0.90 ± 0.19) g respectively. The rate of tumor growth inhibition was 46.76% and 58.33%, respectively. Compared with the model control group, the difference was statistically significant (P <0.05) The VES 100,200 mg.kg-1 could significantly increase the levels of TNF-α and IL-2 in the serum (all P <0.05) . CONCLUSION: VES can significantly inhibit the growth of mouse H22 tumor cells in vivo, and its mechanism may be related to arresting the cell cycle at G0 / G1 phase and up-regulating the expression of TNF-α and IL-2 in serum.