Irradiation Injury Temporarily Induces Enhancement of APN/CD13 Peptidase Activity on Aorta-gonads-me

• 作 者: 朱艳 黄丽芳 罗小华 孙汉英 冉丹 张可杰 郑邈 周琨 刘文励
• 机 构: Department of Hematology Tongji Hospital Tongji Medical School Huazhong University of Science and T
• 出 处: Journal of Huazhong University of Science and Technology(Med
• 发表日期: 2004年期
• 关键词: irradiation hematopoietic enzymatic irradiated creased peptidase ladder returned

This study was designed to investigate the expression of aminopeptidase N (APN)/CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury. The expression of APN/CD13 on AGM stromal cells was assayed by RT-PCR and immunihistochem- istry. After the stromal cells in AGM region were irradiated with 8.0 Gy of 60Co γ-rays, APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN/CD13. The enzymatic activity of APN/CD13 de- creased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. The enzymatic activity of APN/CD13 was temporarily enhanced after irradiation injury, which might be one of the compensa- tory mechanisms that promote the hematopoietic recovery after irradiation.

This study was designed to investigate the expression of aminopeptidase N (APN) / CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury. The expression of APN / CD13 on AGM stromal cells was assayed by RT-PCR and After the stromal cells were irradiated with 8.0 Gy of 60Co γ-rays, APN / CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN / CD13. enzymatic activity of APN / CD13 de- creased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. enhanced after irradiation injury, which might be one of the compensa- tory mechanisms that promote the hematopoietic recovery after irradiation.