论文部分内容阅读
目的分析比较单纯性外阴阴道假丝酵母菌病(VVC)和复发性外阴阴道假丝酵母菌病(RVVC)阴道细菌菌群结构的差异。方法采集VVC患者5例、RVVC患者5例及健康育龄期女性2例的阴道后穹窿分泌物,提取样品中细菌总DNA,扩增16S r RNA V4区基因,比较三组的菌属构成、alpha多样性、beta多样性。结果与健康组相比,VVC患者乳杆菌属比例显著降低,加德纳菌、普氏菌属、双歧杆菌等菌属相对丰富度升高,菌群Alpha多样性也明显增加(P<0.05)。RVVC患者乳杆菌属主要以惰性乳杆菌为优势菌,约占80%。结论 VVC组阴道菌群乳杆菌属比例降低,加德纳菌、普氏菌属、双歧杆菌等菌属丰富度增加。RVVC组以惰性乳杆菌为优势菌。
Objective To compare the vaginal bacterial flora differences between simple vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC). Methods Vaginal posterior fornix secretions were collected from 5 patients with VVC, 5 with RVVC and 2 healthy women of childbearing age. The total bacterial DNA was extracted and the 16S rRNA V4 region was amplified. Diversity, beta diversity. Results Compared with the healthy group, the proportion of Lactobacillus was significantly decreased in VVC patients. The relative abundance of Gardnerella, Prevotella, Bifidobacterium and other bacteria were increased, and the diversity of alphabacteria also increased significantly (P <0.05 ). Lactobacillus in patients with RVVC mainly lactobacillus laxative bacteria, accounting for about 80%. Conclusions The ratio of Lactobacillus in vaginal flora in VVC group is decreased, and the richness of Gardnerella, Prevotella and Bifidobacterium are increased. RVVC group with lactobacillus as dominant bacteria.