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目的 了解广东瑶族无偿献血者人细小病毒B19(HPV B19)感染现状,探讨HPV B19与输血相关肝炎病毒HBV和HCV及HGV的相关性.方法 采用ELISA法分别检测血液中HPV B19 IgG、HBsAg、抗-HCV和抗-HGV-IgG,并用fast real time PCR法检测部分样本中的HPV B19 DNA.结果 376例瑶族无偿献血者检出HPV B19 IgG抗体阳性110例,阳性率29.26％,在抗-HGV-IgG、抗-HCV及HBsAg阳性血液中HPV B19 IgG阳性检出率分别36.36％、33.33％和28.57％,重叠感染达到34.88％,以抗-HGV-IgG阳性检出率最高,与HBsAg及抗-HCV比较差异无统计学意义(x2=0.158,P＞0.05).阴性血液HPV B19 IgG阳性率为28.53％,与标志物阳性血液比较,两者差异无统计学意义(x2=0.743,P＞0.05).HPV B19 DNA阳性率6.80％,在HBsAg、HPV B19 IgG、抗-HGV-IgG、抗-HCV及阴性血液中阳性率分别为14.29％、11.63％、7.69％、0.00％及3.70％,之间差异无统计学意义(x2=3.660,P＞0.05).结论 广东瑶族无偿献血者存在HPV B19感染,HPV B19可以单独感染也可与HBV、HCV、HGV重叠感染.“,”Objective To find out the infection status of HPV B19 in blood donors of Yao ethnic group,and explore the relationship between HPV B19 and HBsAg,HCV,HGV.Methods Anti-HPV B19 IgG,HBsAg,anti-HCV IgG and antiHGV-IgG in serum samples were detected by ELISA.Fast real time PCR was used to detect HPV B19 DNA in some of the samples.Results Of all 376 blood samples,110 were positive in anti-HPV B19 IgG detection.The positive rates of anti-HPV B19 IgG detection among those samples positive for anti-HGV-IgG,anti-HCV and HBsAg were 36.36％,33.33％and 28.57％,respectively,while the co-infection rate was as high as 34.88％.Compared with HBsAg and antiHCV,although positive rate of anti-HGV-IgG was the highest,there was no significant difference among them (x2=0.158,P＞0.05).The anti-HPV B19 IgG positive rate of negative blood samples was 28.53％,as there is no significant difference between it and anti-HPV B19 IgG positive rate of positive blood samples after analysis (x2=0.743,P＞0.05).The overall positiverate of HPV B19 DNA detection was 6.80％ ;among the HBsAg positvie,HPV B19 IgG positive,anti-HGV-IgG positive,anti-HCV positive and negative samples,the HPV B19 DNA positive rates were 14.29％,11.63％,7.69％,0.00％ and 3.70％,respectively.Conclusion HPV B19 infection does exist among blood donors of Yao ethnic group,and some donors were co-infected with HBV,HCV and HGV.