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目的：观察维生素Ｅ（ＶＥ）及其联合维生素Ｃ（ＶＥ＋ ＶＣ）的抗氧化作用、对肝细胞凋亡与增殖的调节作用以及抗氧化过程与细胞凋亡和增殖的关系。方法：昆明鼠随机分为幼龄组（４ 月龄）、老年组（１７ 月龄）包括老年对照组、ＶＥ组、ＶＥ＋ ＶＣ组；以硫代巴比妥酸法和磷钨酸法分别检测肝及血清过氧化脂质（ＬＰＯ），流式细胞术测定肝细胞凋亡率并分析肝细胞周期。结果：老龄组肝及血清ＬＰＯ含量均明显高于幼龄组，补以ＶＥ、ＶＥ＋ ＶＣ后ＬＰＯ含量明显降低，尤以ＶＥ＋ ＶＣ效果明显；小鼠肝细胞凋亡率随月龄增加而明显升高，老龄组补以ＶＥ、ＶＥ＋ ＶＣ后肝细胞凋亡率明显降低；与幼龄组相比，老龄组肝细胞发生Ｇ１ 期阻滞，施以抗氧化处理后，Ｇ０／Ｇ１、Ｓ、Ｇ２＋ Ｍ 各期细胞数均发生逆转性变化。结论：ＶＥ具有明显的抗氧化作用，与ＶＣ联用效果更佳，并可显著降低肝细胞凋亡的发生率，提高肝细胞增殖活力。
Objective: To observe the antioxidant effect of vitamin E (VE) and its combined vitamin C (VE + VC) on the regulation of hepatocyte apoptosis and proliferation, and the relationship between anti-oxidative process and apoptosis and proliferation. Methods: Kunming mice were randomly divided into the young group (4 months old), the aged group (17 months old) including the elderly control group, the VE group and the VE + VC group. The thiobarbituric acid and phosphotungstic acid Liver and serum lipid peroxidation (LPO), flow cytometry of hepatocyte apoptosis rate and analysis of liver cell cycle. Results: The content of LPO in liver and serum of aged group was significantly higher than that of young group. The content of LPO in VE and VE + VC group was obviously decreased, especially in VE + VC group. The rate of hepatocyte apoptosis in mice increased significantly with the increase of age The apoptotic rates of hepatocytes in high and old age groups were significantly decreased after VE and VE + VC supplementation. Compared with young age groups, G1 phase arrest occurred in old age groups, and after G / G1, S, G2 + M each phase cells were reversible changes. CONCLUSION: VE has obvious anti-oxidant effect and better effect with VC, and can significantly reduce the incidence of hepatocyte apoptosis and increase the proliferation activity of hepatocytes.