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目的 :研究肾小管细胞溶酶体损伤在顺铂所致实验性 ARF发病中的作用 ,探讨 8种 L-氨基酸合剂的肾功能保护作用及机理。方法 :雄性 Sprague- Dawley鼠静脉注射 2 ml氨基酸合剂或0 .9% Na Cl溶液后接受 10 mg/ kg顺铂 ,随后氨基酸或 Na Cl溶液以 2 ml/ h维持 3h,测定尿 NAG活性 ,尿钠、钾浓度 ,GFR及作形态学观察。结果 :顺铂注射后 30 min,尿 NAG活性增加 7.8倍 ,尿钠、钾浓度分别增加 56%及 2 .6倍。应用该氨基酸合剂使增高的尿 NAG活性减少 76% ,改善肾小管钠、钾排泄异常 ,增加 GFR 85%。结论 :溶酶体损伤可能在顺铂所致实验性 ARF发生极早期阶段起重要作用 ,氨基酸合剂有明显的肾功能保护作用 ,其机理可能通过改善受损的溶酶体功能所致。
AIM: To investigate the role of renal cell lysosome damage in the pathogenesis of experimental ARF induced by cisplatin and to explore the renal protective effects and mechanisms of 8 L-amino acid mixtures. Methods: Male Sprague-Dawley rats received intravenous injection of 2 ml of amino acid mixture or 0.9% NaCl solution and 10 mg / kg of cisplatin followed by 2 hours of maintenance of amino acid or NaCl solution for 3 hours. Urine NAG activity and urine Sodium, potassium concentration, GFR and morphological observation. RESULTS: Urine NAG activity increased 7.8-fold and urinary sodium and potassium levels increased 56% and 2.6-fold respectively 30 min after cisplatin injection. The application of the amino acid mixture can increase the activity of urinary NAG by 76%, improve renal tubular sodium and potassium excretion, increase GFR 85%. Conclusion: Lysosomal damage may play an important role in the very early stage of experimental ARF induced by cisplatin. Amino acid mixture may have a protective effect on renal function, which may be caused by the improvement of impaired lysosomal function.