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目的研究八氯代二苯并二噁英(OCDD)对C57BL/6j雄性小鼠肝脏结构及Caspase-3表达的影响,探讨其对肝脏的毒性作用。方法将40只7～8周龄SPF级雄性C57BL/6j小鼠按体重随机分为4组,分别为对照组(玉米油)和低(1.25×10-6 g/ml)、中(1.25×10-5 g/ml)、高(1.25×10-4 g/ml)剂量OCDD染毒组,每组10只。采取灌胃方式进行染毒,染毒容量为8 ml/kg,1次/d,连续染毒30 d,染毒结束后观察肝脏体重比值的改变;HE染色观察肝脏的光镜结构;免疫组化法(SP法)检测肝脏组织中Caspase-3的表达。结果染毒30 d后,小鼠肝脏的结构发生了改变,染毒剂量越大结构改变越明显;与对照组比较,各染毒组小鼠肝脏体重比值增加显著(P<0.05);与对照组比较,Caspase-3蛋白在中剂量组和高剂量组表达均增高(P<0.05),其中高浓度组差异有显著统计学意义(P<0.01),且染毒剂量与Caspase-3表达存在剂量-效应关系。结论在本实验条件下,OCDD可导致小鼠肝脏损伤。
Objective To investigate the effects of octachlorinated dibenzo-dioxins (OCDD) on the hepatic structure and Caspase-3 expression in C57BL / 6j male mice and its toxicity to the liver. Methods Forty SPF male C57BL / 6j mice (7-8 weeks old) were randomly divided into 4 groups according to body weight: control group (corn oil) and low (1.25 × 10-6 g / ml) 10-5 g / ml) and high (1.25 × 10-4 g / ml) doses of OCDD. The rats were sacrificed and the rats were sacrificed and the rats were sacrificed. The rats were sacrificed and the rats were sacrificed. The rats were challenged by gavage at a dose of 8 ml / kg once a day for 30 days. The liver weight was observed after exposure. Method (SP method) to detect the expression of Caspase-3 in liver tissue. Results The structure of mouse liver changed after 30 days of exposure. The larger the dose was, the more obvious the structural change was. Compared with the control group, the liver weight ratio increased significantly in each exposure group (P <0.05) (P <0.05). Caspase-3 protein expression was significantly increased in medium dose group and high dose group (P <0.05), and there was significant difference between high dose group and control group (P <0.01) Dose-effect relationship. Conclusion Under the experimental conditions, OCDD can cause liver damage in mice.