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根据对１２种已知抗菌肽的结构－活性相关关系的分析，利用计算机模拟，以天然抗菌肽中杀菌活力最强的ＣｅｃｒｏｐｉｎＢ为蓝本，设计并手工合成了３种对革兰氏阳性及阴性细菌均具有强杀伤活性的新抗菌肽ＡＢＰ３、Ｓｈｉｖａ２Ａ及ＷＨＤ。将抗菌肽ＣｅｃｒｏｐｉｎＢ、ＳｈｉｖａＡ的单价基因ＣｅｃｒｏｐｉｎＢ、ＳｈｉｖａＡ及ＣｅｃｒｏｐｉｎＢ／ＳｈｉｖａＡ双价基因通过农杆菌介导法导入我国７个马铃薯主栽品种（系）中，获得了１０５０个转基因株系。分子检测证明了目的基因在转化植株中的整合与表达。在温室及田间人工病圃对部分转基因株系接种青枯菌进行了抗病性鉴定，从７５个经过多年多点重复鉴定的株系中，筛选得到了３个比起始品种抗病性提高１～３级、达到中抗的株系。
Based on the analysis of the structure-activity relationships of 12 known antimicrobial peptides, computer simulation was used to design and synthesize 3 kinds of Gram-positive and -negative bacteria The new antimicrobial peptides ABP3, Shiva2A and WHD all possess strong killing activity. The bivalent genes CecropinB, ShivaA and CecropinB / ShivaA of antibacterial peptides CecropinB and ShivaA were introduced into 7 potato cultivars (lines) in China by Agrobacterium tumefaciens, and 1050 transgenic lines were obtained. Molecular detection proved the integration and expression of the target gene in the transformed plants. The resistance of R. solanacearum to some of the transgenic lines was evaluated in greenhouse and field nursery. Three of the 75 lines tested for resistance to R. solanacearum 1 to 3, to reach the line of resistance.