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为了探讨脱落酸调控草莓果实成熟的信号识别机制,以‘北农香’草莓花后1~4周的果实为试材,在克隆脱落酸受体基因FaABAR/CHLH的基础上,通过荧光定量PCR研究了果实发育过程中该基因表达量的变化及其影响因素。结果表明,草莓果实中脱落酸受体FaABAR/CHLH基因编码一个含有1381个氨基酸的蛋白,且该蛋白含有一个金属离子螯合酶H亚基超家族保守区。在果实发育的前期(小绿、大绿和浅绿果期),FaABAR/CHLH基因表达量维持较高水平;随着果实的加速褪绿,其表达量迅速降低,至白果期降到最低点;随着果实着色启动,其表达量又迅速上升。ABA和高pH能促进FaABAR/CHLH基因在转录水平上的表达,而蔗糖则抑制该基因转录水平。草莓果实中脱落酸受体基因FaABAR/CHLH表达水平的变化进一步揭示了ABA在调控果实成熟中的重要作用。
In order to investigate the signal recognition mechanism of abscisic acid on the regulation of strawberry fruit ripening, we used the fruit of ’North Nongxiang’ strawberry flower 1 to 4 weeks after flowering as the test material, cloned abscisic acid receptor gene FaABAR / CHLH, The change of gene expression during fruit development and its influencing factors were studied. The results showed that the FaABAR / CHLH gene of abscisic acid receptor in strawberry fruit encoded a protein of 1381 amino acids and contained a conserved region of the H subunit of metal ion chelatase. FaABAR / CHLH gene expression maintained a high level in the early fruit development stage (small green, big green and light green stage); the expression of FaABAR / CHLH gene rapidly decreased with the accelerated chlorosis of fruit, With the start of fruit coloring, its expression increased rapidly. ABA and high pH can promote FaABAR / CHLH gene expression at the transcriptional level, while sucrose inhibited the gene transcription level. The changes of ABA receptor gene FaABAR / CHLH expression in strawberry fruit further revealed the important role of ABA in regulating fruit ripening.