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There has been considerable interest in researching the regulatory mechanisms that control thesynthesis of interleukin (IL)-12,which plays a central role in the differentiation of T-helper-1 cells.In thisstudy,we performed a series of transient transfection experiments designed to elucidate the functional rela-tionship between the IL-12 promoter-specific transcription factors (Ets2 and Sp1) and histone acetylationmodification in IL-12 regulation mediated by p300 and various histone deacetylases (HDACs).Results pre-sented in this report demonstrated that the transcription factors Ets2 and Sp1 acted synergistically with p300to activate the human IL-12 promoter.The histone acetyltransferase (HAT) activity of p300 was required forthis synergic effect,because the adenovirus E1A protein inhibited the synergy.Conversely,HDACs re-pressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12,whilep300 was able to rectify it.These data indicated that Ets2 and Sp1 worked concertedly and synergisticallywith p300 in the regulation of human IL-12 expression.
There has been apparent interest in researching the regulatory mechanisms that control thesynthesis of interleukin (IL) -12, which plays a central role in the differentiation of T-helper-1 cells. This thisudy, we performed a series of transient transfection experiments experiments to elucidate the functional rela-tionship between the IL-12 promoter-specific transcription factors (Ets2 and Sp1) and histone acetylation modification in IL-12 regulation mediated by p300 and various histone deacetylases (HDACs). Results pre-sented in this report that that the the transcription factors Ets2 and Sp1 acted synergistically with p300to activate the human IL-12 promoter.The p300 was required forthis synergic effect, because the adenovirus E1A protein inhibited the synergy. Conversely, HDACs re-pressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12, whilep300 was able to rectify it. 2 and Sp1 worked concertedly and synergistically with p300 in the regulation of human IL-12 expression.