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用制霉菌素穿孔膜片钳方法研究５－ＨＴ和ＮＡ对急性分离的大鼠骶髓后连合核神经元甘氨酸门控氯离子通道电流（ＩＧｌｙ）的调控作用及其胞内机制。发现：（１）５－ＨＴ激活与非胰岛激活蛋白（ＩＡＰ）敏感型Ｇ蛋白偶联的５－ＨＴ２受体亚型，激活磷脂酶Ｃ（ＰＬＣ），增加甘油二酯（ＤＡＧ）的生成。ＤＡＧ增强不依赖Ｃａ２＋的新型ＰＫＣ（ｎＰＫＣ）的活性，从而增强ＩＧｌｙ；（２）ＮＡ激活与ＩＡＰ敏感型Ｇ蛋白偶联的α２受体，抑制腺苷酸环化酶（ＡＣ），减少ｃＡＭＰ的生成，使ＰＫＡ活性降低，从而增强ＩＧｌｙ。
The effect of 5-HT and NA on the regulation of glycine-gated chloride ion current (IGly) in acutely isolated rat sacral spinal cord nucleus and its intracellular mechanism were studied by nystatin-perforated patch-clamp method. We found that: (1) 5-HT activates the 5-HT2 receptor subtype that is coupled with non-islet-activating protein (IAP) -conjugated G-protein and activates phospholipase C (PLC) to increase the production of diglyceride (DAG). DAG enhances the activity of a non-Ca2-dependent novel PKC (nPKC), thereby enhancing IGly; (2) NA activates α2 receptors coupled to IAP-sensitive G proteins, inhibits adenylyl cyclase (AC), decreases cAMP Generate, reduce PKA activity, thereby enhancing IGly.