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为了寻找能准确反映蛔虫感染情况和流行动态的调查方法,我们用ELISA试验定期检测实验感染猪蛔虫卵的家兔的血清抗体水平。 材料和方法 1 抗原 从刚屠宰的猪肠管内收集活的雌蛔虫,用自来水和无菌生理盐水反复洗涤后,收集体腔液,10000rpm离心沉淀30min。上清液经硫酸铵
In order to find a survey method that can accurately reflect the infection status and prevalence of Ascaris infection, we used ELISA test to test serum levels of antibodies in rabbits infected with Ascaris suum eggs. MATERIALS AND METHODS 1 Antigen Lively Ascaris suum was collected from the swine intestine just after slaughter. After repeated washing with tap water and sterile saline, the body cavity fluid was collected and centrifuged at 10,000 rpm for 30 minutes. The supernatant was subjected to ammonium sulfate