论文部分内容阅读
马凡氏综合征(Marfan’s Syndrome,MS)是以累及骨髓、眼和心血管系统等为主要表现的一种遗传性结缔组织疾病.好发于胶原组织含量高的组织器官,其本质为先天性胶原生成和成熟障碍. 本研究采用的苦味酸天狼星红——偏振光方法(Picrosirius Red-polarization)是一种有特色的组织化学方法,可用于观察分析组织内的胶原类型和分布状态.组织切片经苦味酸天狼星红染色后,偏振光显微镜下可见胶原显示不同的干涉色和不同强度的双折光,可区分Ⅰ型和Ⅲ型等胶原.该方法灵敏度高,重复性好,分辨率高. 采用由阜外医院确诊为马凡氏综合征的6例患者的主动脉和皮肤的活检标本,并有正常人相应活检组
Marfan’s Syndrome (MS) is a hereditary connective tissue disease that mainly affects the bone marrow, eye and cardiovascular system, etc. It occurs naturally in tissues and organs with high collagen content and is essentially congenital Collagen formation and maturation disorders Picrosirius Red-polarization method used in this study is a distinctive histochemical method for the observation and analysis of collagen types and distribution in tissues Tissue sections After picric acid Sirius red staining, under the polarized light microscope, the collagen shows different interference colors and birefringence with different intensities, which can distinguish type I and type III collagen, etc. The method has the advantages of high sensitivity, good repeatability and high resolution. A biopsy specimen of the aorta and skin of 6 patients diagnosed as Marfan syndrome by Fu Wai Hospital and corresponding normal biopsy group