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给去垂体大鼠注射促性腺激素释放激素(GnRH)的类似物(GnRHa)可诱导卵巢颗粒细胞(GC)和膜间质细胞(TI)组织型纤溶酶原激活因子(tPA)和抑制因子(PAI-1)基因在时间上和不同细胞间的协调表达。tPA主要由GC产生,GC也分泌少量PAI-1;而卵巢中的PAI-1主要由TI产生,TI也分泌少量tPA。在排卵前GC和TI中tPA mRNA和生物活性均达到高峰;与此相反,PAI-1的表达在GC和TI中却完全不同,TI中的PAI-1 mRNA和生物活性在tPA峰值前6h达到最高水平;而GC中的PAI-1峰值却出现在排卵后。上述变化与人绒毛膜促性腺激素(hCG)诱导正常大鼠排卵所引起的两种基因的表达的动力学变化无异。这些结果提示,hCG(通过PKA途径)和GnRHa(通过PKC途径)这两种不同的调控信号可通过各自不同的受体,经过不同的信息传递系统而最终影响tPA和PAI-1基因的相同的协调表达引起排卵。
Administration of GnRHa (GnRHa) to pituitary rats induced ovarian granulosa cells (GC) and tissue plasminogen activator (tPA) and inhibitor of membrane interstitium (TI) (PAI-1) gene in time and coordinated expression between different cells. TPA is mainly produced by GC, GC also secrete a small amount of PAI-1; PAI-1 in the ovary is mainly produced by TI, TI also secrete a small amount of tPA. On the contrary, the expression of PAI-1 was completely different between GC and TI, and PAI-1 mRNA and biological activity in TI reached the level of 6 h before tPA peak The highest level; while the peak of PAI-1 in GC appears after ovulation. The above changes are similar to the kinetic changes of the expression of the two genes induced by human chorionic gonadotropin (hCG) -induced ovulation in normal rats. These results suggest that the two different regulatory signals hCG (via the PKA pathway) and GnRHa (via the PKC pathway) may ultimately affect the same (eg, the same) tPA and PAI-1 genes through different information delivery systems Coordinated expression causes ovulation.