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目的研究不同质量浓度吲哚丁酸(IBA)和萘乙酸(NAA)以及培养方式对匍枝筋骨草生长和β-蜕皮甾酮量的影响。方法以MS为基本培养基,添加IBA(0.5、1.0、2.0 mg/L)或NAA(0.3、0.6、0.9 mg/L)进行组织培养,HPLC法测定匍枝筋骨草中β-蜕皮甾酮量。并比较不同生长调节剂以及组织培养、水培不同培养方式下匍枝筋骨草生根情况与β-蜕皮甾酮量的变化。结果以MS为基本培养基,添加生长调节剂IBA0.5～1 mg/L时,适宜匍枝筋骨草生根。IBA质量浓度为2 mg/L时,匍枝筋骨草根中β-蜕皮甾酮量最高。组织培养条件下,匍枝筋骨草全株及根部的蜕皮激素量均达水培苗的2倍,实生苗的3倍。结论适宜适量浓度的IBA可以促进匍枝筋骨草根的生长,组织培养条件下,匍枝筋骨草根中β-蜕皮甾酮量最高。
Objective To study the effects of different concentrations of indole butyric acid (IBA) and naphthalene acetic acid (NAA) and their culture methods on the growth and β-ecdysterone content of Cunninghamia lanceolata. Methods MS was used as the basic medium and IBA (0.5,1.0,2.0 mg / L) or NAA (0.3,0.6,0.9 mg / L) was added to the tissue culture. The content of β-ecdysterone . The changes of rooting amount and β-ecdysterone in different growth regulators, tissue culture and hydroponic culture were compared. Results With MS as the basic medium, when the growth regulator IBA 0.5 ~ 1 mg / L was added, the root of Cunninghamia lanceolata was suitable. When the concentration of IBA was 2 mg / L, the content of β-ecdysterone was the highest in Cunninghamia lanceolata. Under tissue culture conditions, the amount of ecdysone in the whole plant and root of Cunninghamia lanceolata reached 2 times of that of hydroponic seedling and 3 times of seedling. Conclusion The appropriate concentration of IBA can promote the growth of Cunninghamia lanceolata root. Under tissue culture, the content of β - ecdysterone in Creeping bone grass is the highest.