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目的建立简单高效的原位膀胱癌动物模型,探讨引入TA(tumor area)参数来量化分析评价肿瘤发展。方法应用多聚赖氨酸预处理C57BL/6小鼠膀胱,建立MB49小鼠原位膀胱癌动物模型;实验组予以丝裂霉素灌注,对照组予以PBS灌注,检验模型在药物实验中的可靠性。取小鼠膀胱组织标本行HE染色进行病理学检查,利用肿瘤面积占膀胱总体面积百分数TA来评价肿瘤发展。结果多聚赖氨酸预处理膀胱黏膜小鼠原位膀胱癌模型成瘤率接近100%,肿瘤生长基本模拟了人膀胱癌的发生、发展过程;丝裂霉素灌注能显著降低膀胱重量及TA。结论多聚赖氨酸预处理小鼠膀胱可建立简单高效的原位膀胱癌动物模型,基本模拟了人膀胱癌的发生、发展过程。TA可对膀胱肿瘤进行准确量化分析。
Objective To establish a simple and efficient animal model of bladder cancer in situ and to explore the introduction of TA (tumor area) parameters to quantitatively evaluate the tumor development. Methods The bladder of C57BL / 6 mice was pretreated with polylysine to establish an animal model of bladder cancer in situ in MB49 mice. The experimental group was given mitomycin C and the control group was infused with PBS. The model was reliable in drug experiment Sex. Tissue specimens of mice bladder were examined by HE staining for pathological examination, and the tumor area was assessed by the area of the tumor as a percentage of the total area of the bladder. Results Polylysine pre-treatment of bladder mucosa in situ bladder cancer model of tumor formation rate of nearly 100%, tumor growth basically simulate the occurrence and development of human bladder cancer; mitomycin infusion can significantly reduce bladder weight and TA . Conclusions Polylysine preconditioning in mouse bladder can establish a simple and efficient animal model of bladder cancer in situ and basically simulate the occurrence and development of human bladder cancer. TA can accurately quantify the bladder cancer.