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目的研究肝再生磷酸酶3(PRL-3)在胶质瘤细胞SHG-44中作用。方法采用siRNA干涉的方法下调SHG-44中PRL-3表达水平,通过Western blot方法检测干涉效果,绘制细胞生长曲线、流式细胞仪检测细胞周期及凋亡以观察下调PRL-3对SHG-44细胞的影响。结果 Western blot提示siRNA能够下调PRL-3在SHG-44细胞中的表达,细胞生长曲线显示下调PRL-3能够抑制细胞的增殖,流式细胞仪检测发现下调PRL-3的表达可以促进SHG-44细胞G1期阻滞,S期减少,凋亡增加。结论下调PRL-3能够明显抑制SHG-44细胞的增殖,PRL-3很可能成为脑胶质瘤基因治疗的新靶点。
Objective To study the role of liver regeneration phosphatase 3 (PRL-3) in glioma cells SHG-44. Methods The expression of PRL-3 in SHG-44 was down-regulated by siRNA interference. The interference effect was detected by Western blotting. The cell growth curve was drawn. The cell cycle and apoptosis were detected by flow cytometry to observe the effect of PRL-3 on SHG-44 Effects of cells. Results Western blot showed that siRNA could downregulate the expression of PRL-3 in SHG-44 cells. The cell growth curve showed that down-regulation of PRL-3 could inhibit the proliferation of SHG-44 cells. Flow cytometry showed that downregulation of PRL- Cell G1 arrest, S phase decreased, apoptosis increased. Conclusion PRL-3 downregulation can significantly inhibit the proliferation of SHG-44 cells, PRL-3 is likely to become a new target for gene therapy of glioma.