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目的:建立测定氨茶碱血清浓度的方法。方法:采用反相高效液相色谱法,色谱柱为BDS C18,流动相为甲醇-水(15∶85),柱温为35 ℃,流速为1 mL·min-1,检测波长为275 nm。结果:氨茶碱有效成分茶碱血药浓度在0.5～42.5 μg·mL-1范围内线性关系良好(r=0.999 9),最低检测限为0.5 μg·mL-1;方法回收率为91.60%～95.00%,提取回收率为68.04%～70.77%;日内RSD≤2.20%,日间RSD≤3.28%。结论:本方法简便、准确、灵敏、重复性好,可用于氨茶碱血药浓度监测。
Objective: To establish a method for determination of serum concentration of aminophylline. Methods: The mobile phase was methanol-water (15:85). The column temperature was 35 ℃, the flow rate was 1 mL · min-1 and the detection wavelength was 275 nm by reversed-phase high performance liquid chromatography. Results: The linear range of theophylline active ingredient theophylline ranged from 0.5 to 42.5 μg · mL-1 (r = 0.999 9), the lowest limit of detection was 0.5 μg · mL-1. The recovery rate was 91.60% ~ 95.00%, the recovery rate was 68.04% ~ 70.77%; RSD≤2.20%, daytime RSD≤3.28%. Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used to monitor the plasma concentration of aminophylline.