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目的:探讨中药益气健脾方对糖尿病胃轻瘫大鼠的疗效。方法:SD大鼠被随机均分为正常对照组、模型对照组、益气健脾方低剂量组、益气健脾方高剂量组和西药组。腹腔注射STZ建立大鼠糖尿病胃轻瘫模型,益气健脾方低、高剂量组和西药组分别用低、高剂量益气健脾方和西药吗丁啉混悬液灌胃,正常对照组和模型对照组均给予等量蒸馏水灌胃。检测比较各组胃蠕动功能,用酚红灌胃实验测定胃排空率,用免疫组化法检测胃窦肌间c-kit阳性ICC的含量。结果:益气健脾方低、高剂量组和西药组大鼠胃蠕动的频率和幅度、胃排空率及胃窦ICC含量均高于模型对照组(P<0.05),且随着益气健脾方浓度的增高,胃排空率、胃窦ICC含量改善更加明显,差异有统计学意义(P<0.05);益气健脾方高剂量组与西药组差异无统计学意义(P>0.05)。结论:中药益气健脾方对糖尿病胃轻瘫大鼠有明确疗效,其机制可能是通过改善胃窦ICC含量来实现的。
Objective: To investigate the curative effect of Yiqi Jianpi prescription on diabetic gastroparesis in rats. Methods: SD rats were randomly divided into normal control group, model control group, low dose Yiqi Jianpi Decoction, high dose Yiqijianpi Decoction group and Western medicine group. The model of diabetic gastroparesis was established by intraperitoneal injection of STZ in rats. The model of Qi-qi and spleen-qi was low. The high-dose and high-dose of Yiqi Jianpi Decoction And model control group were given equal volume of distilled water gavage. The gastric motility of each group was detected and compared. The gastric emptying rate was measured by gingivol red gavage, and the content of ICC in antral pylorus was detected by immunohistochemistry. Results: The frequency and amplitude of gastric motility, gastric emptying rate and ICC content of gastric antrum in Yiqianjianpi Decoction low, high dose and western medicine groups were higher than those in model control group (P <0.05) There was no significant difference between the high-dose group and the western medicine group (P> 0.05), and the difference was statistically significant (P < 0.05). Conclusion: The traditional Chinese medicine Yiqi Jianpi Decoction has a definite curative effect on diabetic gastroparesis in rats. The mechanism may be achieved by improving the content of ICC in gastric antrum.