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目的:研究外周血CD4+CD25+Tr细胞的比例变化,探讨CD4+CD25+Tr细胞在宫颈癌患者外周血中的表达特点及临床意义。方法:应用流式细胞术检测30例宫颈鳞癌,60例宫颈上皮内瘤变及30例正常妇女外周血中CD4+CD25+Tr细胞的比例。结果:30例宫颈癌患者外周血中CD4+CD25+Tr细胞比例明显高于健康对照组(P<0.01);随着疾病进展,宫颈癌患者外周血中CD4+CD25+Tr细胞比例升高,分别高于CINⅠ期、CINⅡ期、CINⅢ期;CINⅢ期患者外周血中CD4+CD25+Tr细胞比例明显高于CINⅠ期患者、CINⅡ期和健康对照组(P<0.05);CINⅠ期患者外周血CD4+CD25+Tr细胞比例与健康对照组相比差异无统计学意义(P>0.05)。结论:宫颈癌患者外周血CD4+CD25+Tr细胞表达水平可能是一个较好的反映宫颈癌患者免疫功能状态的参考指标,其在外周血的升高程度与肿瘤临床分期有关,可能参与了宫颈癌的发生发展过程。
Objective: To study the changes of the proportion of CD4 + CD25 + Tr cells in peripheral blood and to explore the characteristics and clinical significance of the expression of CD4 + CD25 + Tr cells in peripheral blood of patients with cervical cancer. Methods: Flow cytometry was used to detect the proportion of CD4 + CD25 + Tr cells in peripheral blood of 30 cases of cervical squamous cell carcinoma, 60 cases of cervical intraepithelial neoplasia and 30 cases of normal women. Results: The proportion of CD4 + CD25 + Tr cells in peripheral blood of 30 patients with cervical cancer was significantly higher than that of healthy controls (P <0.01). With the progression of disease, the proportion of CD4 + CD25 + Tr cells in peripheral blood of patients with cervical cancer increased, Respectively, which were higher than those in CINⅠ, CINⅡand CINⅢ. The proportion of CD4 + CD25 + Tr cells in peripheral blood of patients with CIN Ⅲ was significantly higher than that of patients with CINⅠ, CINⅡ and healthy controls (P <0.05) + CD25 + Tr cells compared with the healthy control group, the difference was not statistically significant (P> 0.05). Conclusion: The expression of CD4 + CD25 + Tr cells in peripheral blood of patients with cervical cancer may be a good reference index to reflect the immune status of patients with cervical cancer. The increase in peripheral blood is related to the clinical stage of the cancer and may be involved in the cervical The occurrence and development of cancer.