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目的:研究高渗透压奶粉溶液对乙二醇和氯化铵诱导的大鼠肾结石形成的影响。方法:40只21日龄健康雄性断乳SD大鼠按体重随机分为空白对照组、模型对照组、低渗透压奶粉溶液实验组、高渗透压奶粉溶液实验组;模型对照组及实验组使用1%乙二醇和氯化铵联合造模方法建立大鼠肾脏结石模型,实验组灌胃(2 ml/100 gbw,Bid)给予低渗透压和高渗透压奶粉溶液,模型和空白对照组以同样方式给予等体积蒸馏水,实验周期7周。实验结束前测定24 h尿量;实验结束时检测血清肌酐、尿素氮水平;观察肾脏草酸钙结晶形成情况。结果:乙二醇和氯化铵联合应用可引起大鼠肾脏损伤,并诱导肾脏草酸钙结石。实验期间,给予高渗透压奶粉溶液的大鼠体重增长明显受到抑制;24 h尿量、血清尿素氮、肌酐水平、肾脏/体重比值明显高于模型对照组(P<0.05),且24 h尿量、血清肌酐、肾脏草酸钙结晶评分明显高于低渗透压奶粉溶液实验组(P<0.05)。结论:在本实验条件下,高渗透压奶粉溶液对乙二醇和氯化铵诱导的肾结石形成有明显的促进作用,并且这一作用可能与其中的无机盐含量较高有关。
Objective: To study the effects of high osmotic pressure milk powder on the formation of rat kidney stones induced by ethylene glycol and ammonium chloride. Methods: Forty healthy 21-day-old male weanling SD rats were randomly divided into blank control group, model control group, low osmotic pressure milk powder test group and high osmotic pressure milk powder test group; model control group and experimental group 1% ethylene glycol and ammonium chloride modeling method to establish rat kidney stone model, the experimental group by intragastric administration (2 ml / 100 gbw, Bid) given low osmotic pressure and high osmotic pressure milk powder solution, the model and the blank control group with the same Way to give equal volume of distilled water, the experimental period of 7 weeks. Determination of 24 h urine output before the end of the experiment; serum creatinine and urea nitrogen levels were measured at the end of the experiment; and kidney calcium oxalate crystal formation was observed. Results: The combined application of ethylene glycol and ammonium chloride can cause kidney damage in rats and induce renal calcium oxalate stones. During the experiment, the weight gain of rats fed with high osmotic pressured milk powder solution was significantly inhibited. The 24 h urinary output, serum urea nitrogen, creatinine level and kidney / body weight ratio were significantly higher than those of model control group (P <0.05) The levels of serum creatinine and kidney calcium oxalate were significantly higher than those of low osmotic pressure milk powder (P <0.05). Conclusion: Under the experimental conditions, high osmotic pressure milk powder solution can significantly promote the formation of kidney stones induced by ethylene glycol and ammonium chloride, and this effect may be related to the high content of inorganic salts therein.