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目的:研究选择性β2肾上腺素能受体激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外由大鼠骨髓间充质干细胞(MMSCs)诱导分化的成骨样细胞增殖和凋亡的影响,探讨β2肾上腺素能受体信号对骨代谢的影响。方法:取3周龄雌性SD大鼠,全贴壁筛选法培养MMSC,用条件培养液诱导向成骨细胞分化后分别加入不同浓度Formoterol和ICI118551,检测细胞增殖率和细胞凋亡率。结果:MMSC成功诱导分化为成骨样细胞,不同浓度(10-5～10-9mmol/L)的Formoterol均可抑制成骨样细胞增殖。高浓度ICI118551(10-5和10-6mmol/L)抑制成骨样细胞细胞增殖,低浓度ICI118551(10-8和10-9mmol/L)则可促进成骨样细胞增殖。不同浓度(10-5～10-9mol/L)的福莫特罗和ICI118551对成骨样细胞的凋亡无明显影响。结论:β2肾上腺素能受体激动剂可抑制体外大鼠成骨样细胞增殖,而阻滞剂对成骨样细胞增殖的影响与其浓度有关。β2肾上腺素能受体激动剂和阻滞剂对大鼠成骨样细胞凋亡无明显影响。
AIM: To investigate the effects of Formoterol, a selective β2 - adrenergic receptor agonist and ICI118551, on the proliferation and apoptosis of osteoblast - like cells induced by rat bone marrow mesenchymal stem cells (MMSCs) in vitro Influence, to explore β2 adrenergic receptor signal on bone metabolism. Methods: Three-week-old female Sprague-Dawley rats were used to culture MMSC. The conditioned medium was used to induce osteoblast differentiation. After different concentrations of Formoterol and ICI118551 were added respectively, the cell proliferation rate and apoptosis rate were measured. Results: MMSC successfully differentiated into osteoblast-like cells. Formoterol with different concentrations (10-5 ~ 10-9mmol / L) could inhibit osteoblast-like cell proliferation. High concentrations of ICI118551 (10-5 and 10-6mmol / L) inhibited osteoblast-like cell proliferation. ICI118551 (10-8 and 10-9mmol / L) could promote osteoblast-like cell proliferation. Different concentrations (10-5 ~ 10-9mol / L) of formoterol and ICI118551 had no significant effect on the apoptosis of osteoblast-like cells. CONCLUSION: β2 adrenergic receptor agonist can inhibit the proliferation of osteoblast-like cells in vitro, while the effect of blockers on the proliferation of osteoblast-like cells is related to its concentration. β2 adrenergic receptor agonists and blockers had no significant effect on the apoptosis of osteoblast-like cells in rats.