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作者应用荧光逆行标记和乙酰胆碱酯酶组织化学相结合技术,研究了大白鼠伏隔核乙酰胆碱酯酶阳性纤维的脑干起源。向一侧伏隔核注入荧光示踪剂二盐酸4,6—双脒基-2—苯吲哚(DAPI)或樱草黄(Pr)。处死前6～8小时肌注二异丙基氟磷酸(DFP)。用含10%福尔马林的磷缓液灌流,连续冠状冰冻切片,Karnovsky—Roots法孵育,荧光显微镜和普通光学显微镜观察同一切片。结果发现,下列核(区)内视到胞体,
The authors used fluorescent retrograde labeling combined with acetylcholinesterase histochemistry to study the brainstem origin of acetylcholinesterase-positive fibers in the nucleus accumbens of rats. Fluorescent tracer dihydrochloride 4,6-bisamidino-2-phenylindole (DAPI) or primrose (Pr) was injected into one of the nucleus accumbens. Diisopropylfluorophosphate (DFP) was intramuscularly administered 6 to 8 hours before sacrifice. Perfusion was performed with phosphate buffered saline containing 10% formalin, serial coronal frozen section, Karnovsky-Roots method, and the same sections were observed by fluorescence microscopy and optical microscopy. The results showed that the following nuclear (area) to the body of cells,