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目的:观察枸杞提取物对MPP+诱导的线虫和PC12细胞神经毒性的保护作用并探讨其作用机制。方法:以不同浓度枸杞提取物预处理MPP+诱导的线虫和PC12细胞多巴胺能神经损伤模型,计算线虫的存活率和选择性表达绿色荧光的多巴胺能退行性变神经元的数量,以MTT法和核形态检测PC12细胞的损伤,检测PC12细胞的胞内的氧自由基、线粒体膜电位、总GSH水平。结果:枸杞提取物明显提高了MPP+处理过的线虫的存活率和保护了多巴胺能神经元,对MPP+诱导的PC12细胞的具有明显的保护作用,作用呈量效关系。枸杞提取物通过减少MPP+诱导PC12细胞胞内ROS的过量积聚,减少线粒体损伤,恢复GSH水平而发挥其保护作用。结论:枸杞提取物主要通过抗氧化及恢复GSH水平而发挥其对MPP+诱导的线虫和PC12细胞神经毒性保护作用。
Objective: To observe the protective effect of Lycium barbarum extract on the neurotoxicity of MPP + -induced nematodes and PC12 cells and to explore its mechanism. Methods: The MPP + -induced nematodes and PC12 cells were pretreated with different concentrations of Lycium barbarum extract, and the survival rate of nematodes and the number of dopaminergic degenerative neurons that selectively expressed green fluorescence were calculated. MTT assay and nuclear The morphological changes of PC12 cells were detected, and the intracellular oxygen free radicals, mitochondrial membrane potential and total GSH levels were detected. Results: Lycium barbarum extract significantly increased the survival rate of MPP + -treated nematodes and protected the dopaminergic neurons. It had a significant protective effect on MPP + -induced PC12 cells in a dose-effect relationship. Goji berry extracts play a protective role by decreasing the excessive accumulation of ROS in PC12 cells induced by MPP +, reducing mitochondrial damage and restoring GSH levels. CONCLUSION: Wolfberry extract exerts its neuroprotective effects on MPP + -treated nematodes and PC12 cells mainly through antioxidation and GSH recovery.