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目的:研究NMDA(N-methyl-D-aspartic acid)和非NMDA受体在介导脊髓内脏痛传入中的作用,方法:气球膨胀(3-15 kPa,20 s)麻醉猫结-直肠诱发脊髓背角痛敏神经元发放,结果:1)扩张结-直肠引起神经元发放增加的为兴奋性型:17个SLA型(短潜伏期突然增加);11个SLS型(短潜伏期渐增);9个LL型(长潜伏期),15个神经元属于抑制性的Inh型,2)67.6％,78.4％和59.5％的膨胀肠诱发兴奋的神经元,分别被微电泳NMDA、使君子酸(QA)和海人藻酸(KA)激活;60％,86.7％和53.3％的Inh神经元也分别被3个酸激活.3)微电泳NMDA受体拮抗剂d,l-2-amino-5-phosphonovalemte(APV)和非NMDA受体拮抗剂6,7-dinitro-quinoxaline-2,3-dione(DNQX),分别使兴奋性反应减少35％±10％和65％±14％,DNQX明显强于APV(P<0.05).DNQX使3/7个Inh神经元抑制翻转30％-50％,而APV无效,结论:NMDA和非NMDA受体均参与介导脊髓内脏伤害性信息传递,而非NMDA受体的作用更强。 (责任编辑 李颖)
OBJECTIVE: To study the role of NMDA (N-methyl-D-aspartic acid) and non-NMDA receptors in mediating spinal visceral pain. Methods: Cat nodal-rectal induction was induced by balloon dilatation (3-15 kPa, 20 s) Spinal dorsal hyperalgesia neurons, the results: 1) dilatation - rectal neuronal firing caused by increased excitatory type: 17 SLA type (a sudden increase in short latency); 11 SLS type (short latency increase); Nine LL type (long incubation period), 15 neurons belong to inhibitory Inh type, 2) 67.6%, 78.4% and 59.5% were induced by dilated intestine and stimulated by NMDA, QA ) And kainic acid (KA), while 60%, 86.7% and 53.3% of Inh neurons were also activated by three acids, respectively.3) Microelectrophoresis of NMDA receptor antagonist d, l-2-amino-5- DNQX was significantly higher than that of phosphoralalterem (APV) and non-NMDA receptor antagonist 6,7-dinitro-quinoxaline-2,3-dione (DNQX), reducing excitatory responses by 35% ± 10% and 65% ± 14% APV (P <0.05) .DNQX inhibited the turnover of 3/7 Inh neurons by 30% -50%, while APV was not effective. Conclusion: NMDA and non-NMDA receptors are involved in the transmission of visceral nociceptive information rather than NMDA The role of the receptor stronger. (Editor Li Ying)