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目的 :研究肾上腺素能受体激动剂对机体细胞免疫功能的作用及下丘脑 垂体 肾上腺轴 (HPA轴 )在此作用中的地位。方法 :以刀豆蛋白A(ConA)刺激脾淋巴细胞的增殖活性为细胞免疫功能指标 ,检测在正常及去肾上腺大鼠海马内注射去甲肾上腺素 (noradrenaline,NA)对机体细胞免疫功能的影响。结果 :①在正常大鼠 ,NA(4μl,6 .0× 10 -3 mol/L)、β1受体激动剂杜丁胺 (dobutamine,Dob ,4 μl,6 .0× 10 -3 mol/L)和 β2 受体激动剂异丙喘宁(metaproterenol,Met,4 μl,8.0× 10 -3 mol/L)均可抑制ConA刺激的脾淋巴细胞增殖反应、降低NK细胞的活性 ,其中NA的作用最强 ,Met次之 ,Dob的作用最弱。α及 β受体阻断剂酚妥拉明 (Phen ,2 μl,1.6× 10 -2 mol/L)和心得安 (Prop ,2 μl,1.6× 10 -2 mol/L)均可部分阻断NA的免疫抑制作用 ,且Prop的作用较强。②在去肾上腺组 ,NA的免疫抑制作用不明显。结论 :海马内NA对机体的细胞免疫功能具有明显的抑制作用 ,此作用由α及 β受体共同介导 ,其中 β受体的作用大于α受体 ,且 β2 受体的作用大于 β1受体。此外 ,保持肾上腺结构和功能完整在NA调节机体细胞免疫功能作用中具有重要意义。
Objective: To investigate the effects of adrenergic receptor agonists on cellular immune function and the role of the hypothalamic pituitary-adrenal axis (HPA axis) in this role. Methods: The proliferative activity of splenic lymphocytes stimulated by ConA was determined as cellular immune function, and the effect of noradrenaline (NA) injection on hippocampal hippocampus cell immune function was examined . Results: ① In normal rats, NA (4μl, 6 × 10-3 mol / L), β1 receptor agonist dobutamine (Dob, 4 μl, 6 × 10-3 mol / L ) And β2 receptor agonist protoporphyrin (Met, 4 μl, 8.0 × 10 -3 mol / L) all inhibited the proliferation of spleen lymphocytes stimulated by ConA and decreased the activity of NK cells. The effect of NA The strongest, Met second, Dob the weakest. Phentolamine (Phen, 2 μl, 1.6 × 10 -2 mol / L) and Prop (2 μl, 1.6 × 10 -2 mol / L) were partially blocked by α and β receptor antagonists NA immunosuppressive effect, and the role of Prop stronger. ② in the adrenal gland, NA immunosuppressive effect is not obvious. CONCLUSION: NA in the hippocampus significantly inhibits the cellular immune function of the body. This effect is mediated by α and β receptors, in which β receptors act more than α receptors and β 2 receptors play a greater role than β 1 receptors . In addition, maintaining adrenal structure and functional integrity plays an important role in the regulation of cellular immune function by NA.