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对具有不同抗性水平的３个西瓜品种进行了离体叶人工接种炭疽病抗性鉴定方法的研究。结果表明，离体叶与幼苗人工接种同样能够真实地反映西瓜品种固有的抗病性，且能节省菌液、劳力、试材和费用，并对环境条件更易控制，简便易行，可以广泛采用。该法用注射器悬滴接种，菌液浓度为１×１０￣６孢子／ｍＬ。接种叶片的叶龄为１５～２０ｄ，接种后３ｄ开始发病，第６ｄ调查统计病情指数。品种抗病性可以用平均病斑直径（ＡＤ），（发病病斑直径）／接种总滴数］和病情指数（ＤＩ）两种标准进行评价，其中用ＡＤ进行品种抗病性评价减少病级划分中的人为误差，更为客观简便，可以取代ＤＩ在离体叶人工接种西瓜炭疽病抗性鉴定中广泛采用。
Three watermelon cultivars with different resistance levels were used to study anthracnose resistance in vitro inoculation. The results showed that artificial inoculation of detached leaves and seedlings can also truly reflect the inherent disease resistance of watermelon cultivars, and can save bacteria, labor, test materials and costs, and the environmental conditions easier to control, easy to use, can be widely used . The law by syringe droplet inoculation, bacterial concentration of 1 × 10 ~ 6 spores / mL. Inoculation leaves leaf age 15 ~ 20d, 3 days after inoculation onset, the first 6d survey statistics disease index. The disease resistance of varieties can be evaluated by two criteria: average lesion diameter (AD), (lesion diameter) / total inoculation amount] and disease index (DI), of which, disease resistance was evaluated by AD The division of the human error, more objective and simple, can replace the DI in vitro artificial identification of watermelon anthracnose inoculation widely used.