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目的 探讨候选的抑癌基因FHIT外显子 5在胃癌中纯合性缺失与突变的情况。方法 用PCR方法研究外显子 5的纯合性缺失 ,用PCR SSCP研究外显子 5的点突变。结果 在 32例原发性胃癌中外显子 5的纯合性缺失率为9 38% (3/ 32 ) ,没发现外显子 5存在点突变。结论 FHIT外显子 5的缺失在胃癌中不一定占主导地位
Objective To investigate the homozygous deletions and mutations of the candidate tumor suppressor gene FHIT exon 5 in gastric cancer. Methods The homozygous deletion of exon 5 was studied by PCR and the point mutation in exon 5 was studied by PCR SSCP. Results The homozygous deletion rate of exon 5 in 32 cases of primary gastric cancer was 9 38% (3/32). No point mutation in exon 5 was found. Conclusion The deletion of FHIT exon 5 may not be dominant in gastric cancer