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目的:探讨六价铬[Cr(Ⅵ)]对人角质形成细胞(HaCaT)的DNA损伤情况及叶酸的干预作用。方法:MTT法检测Cr(Ⅵ)及叶酸对细胞增殖活力的影响,选取接近IC50的Cr(Ⅵ)10.00μmol/L浓度作为染毒组,染毒前以不同浓度叶酸(80、160、320 nmol/L)作为干预组,正常条件培养的细胞作为对照组。单细胞凝胶电泳法(SCGE)检测细胞DNA尾长(μm)、尾部DNA含量(%)和尾矩。结果:Cr(Ⅵ)作用HaCaT细胞24 h的IC_(50)值为15.88μmol/L;DNA损伤比较,染毒组较对照组增加(P<0.05),160、320 nmol/L叶酸干预组较染毒组低(P<0.05),320 nmol/L叶酸干预组DNA损伤与对照组比较差异无统计学意义(P>0.05)。结论:Cr(Ⅵ)对HaCaT细胞有毒作用并可造成细胞DNA损伤,叶酸可减轻并扭转Cr(Ⅵ)所致的细胞DNA损伤。
Objective: To investigate DNA damage induced by Cr (Ⅵ) in human keratinocytes (HaCaT) and the intervention of folic acid. Methods: The effects of Cr (Ⅵ) and folic acid on cell proliferation were detected by MTT assay. The concentration of Cr (Ⅵ) 10.00 μmol / L close to IC50 was chosen as the exposure group. Different concentrations of folic acid (80,160,320 nmol / L) as the intervention group, cells cultured under normal conditions as a control group. Cell tail length (μm), tail DNA content (%) and tail moment were determined by single cell gel electrophoresis (SCGE). Results: The IC 50 value of HaCaT cells treated with Cr (Ⅵ) for 24 h was 15.88 μmol / L. Compared with the DNA damage control group, the IC50 of HaCaT cells increased (P <0.05) Compared with the control group, the DNA damage in 320 nmol / L folic acid intervention group was not significantly different (P> 0.05). CONCLUSION: Cr (Ⅵ) has toxic effect on HaCaT cells and can cause DNA damage. Folic acid can reduce and reverse DNA damage caused by Cr (Ⅵ).