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为研究哺乳期小鼠阿特拉津染毒对仔鼠精子存活率的影响,将16窝健康清洁级昆明小鼠[16只母鼠及其生后7d雄性仔鼠(每窝5～7只)]随机分为4组,分别为对照组(植物油)和低(50mg/kg)、中(100mg/kg)、高(200mg/kg)剂量阿特拉津染毒组,每组4窝。对母鼠进行灌胃染毒,染毒剂量为10ml/kg,每天1次,连续进行14d。仔鼠于生后7～20d通过母乳染毒阿特拉津,生后21～45d自由饲养。各窝母鼠分别于染毒0、7、14d称重;仔鼠于生后9、15、28d称重,称量睾丸重量,并计算脏器系数。在仔鼠生后45d,测定仔鼠附睾中精子的存活率。结果显示,染毒阿特拉津后,各组母鼠体重均略有下降,但差异无统计学意义。各组仔鼠的体重、睾丸重量及其睾丸系数间比较,差异均无统计学意义。与对照组比较,中、高剂量阿特拉津染毒组仔鼠精子存活率较低,差异均有统计学意义(P<0.05);且随着阿特拉津染毒剂量的升高,仔鼠精子存活率呈下降趋势。表明阿特拉津可以通过母鼠乳汁,降低小鼠的精子存活率。
In order to study the effect of atrazine on the sperm viability in lactation mice, 16 healthy and clean Kunming mice [16 males and their male offspring (5-7 rabbits per litter) ) Were randomly divided into 4 groups: control group (vegetable oil) and low (50mg / kg), medium (100mg / kg) and high (200mg / kg) doses of atrazine. Gavage on the mother rats, exposure dose of 10ml / kg, 1 day, for 14 days. Offspring 7 to 20 days after birth by exposure to atrazine in breast milk, born 21 ~ 45d free to feed. The females were weighed at 0, 7 and 14 d after exposure respectively. The offspring were weighed at 9, 15 and 28 days after birth and the weight of testes was weighed and the organ coefficients were calculated. 45 days after birth, the sperm motility in the epididymis of the offspring was measured. The results showed that after exposure to atrazine, the weight of maternal mice in each group decreased slightly, but the difference was not statistically significant. There was no significant difference in body weight, testicular weight and testicular coefficient in each group. Compared with the control group, the sperm survival rate of the offspring of medium and high doses of atrazine was lower, the difference was statistically significant (P <0.05); and with the increase of atrazine dose, Offspring sperm survival rate showed a downward trend. This indicates that atrazine can reduce the sperm viability of mice via lactating maternal milk.