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目的 探讨内皮素 (ET) - 1m RNA和原癌基因 bcl- 2在大鼠内毒素性休克肺损伤中的作用及山莨菪碱、地塞米松对肺的保护作用。 方法 2 4只 SD大鼠随机分为 4组 (n=6 ) : 组 (对照组 )静注等量生理盐水 ; 组 (休克组 )静脉注射脂多糖 (L PS) 5 m g/kg; 组静注山莨菪碱 4m g/kg后 0 .5 h再静注 L PS 5 m g/kg; 组静注地塞米松 4mg/kg后 0 .5 h再静注 L PS 5 mg/kg。观察 5 h后取肺组织测定 ET- 1m RNA和 bcl- 2。 结果 肺组织 ET- 1m RNA表达 , 、 组与 组比较差异无显著性 , 组与 、 、 组比较差异有非常显著性 (P<0 .0 1)。bcl- 2阳性细胞率 组轻微增加 , 、 组显著增加 , 组阴性表达。 结论 ET- 1m RNA和 bcl- 2可介导大鼠内毒素性休克时肺的损伤。山莨菪碱和地塞米松可能是通过抑制 ET- 1m RNA的表达和促进 bcl- 2的表达而起到对肺的保护作用。
Objective To investigate the role of endothelin (ET) - 1 mRNA and proto - oncogene bcl - 2 in lung injury induced by endotoxic shock in rats and the protective effect of Anisodamine and dexamethasone on lung. Method 2 4 SD rats were randomly divided into 4 groups (n = 6): group (control group) were injected with normal saline; group (shock group) were injected with lipopolysaccharide (LPS) 5 mg / kg; Injection of anisodamine 4 m g / kg 0.5 h after intravenous injection of L PS 5 mg / kg; group dexamethasone 4mg / kg 0.5 h after intravenous injection of L PS 5 mg / kg. After 5 h, the lung tissues were removed for determination of ET-1 mRNA and bcl-2. Results The expression of ET-1mRNA in lung tissue was not significantly different between the two groups (P <0.01). There was a significant difference between the two groups (P <0.01). The rate of bcl-2 positive cells increased slightly, the group increased significantly, the group negative expression. Conclusions ET-1m RNA and bcl-2 can mediate lung injury during endotoxic shock in rats. Anisodamine and dexamethasone may protect the lung by inhibiting the expression of ET-1 mRNA and promoting the expression of bcl-2.