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目的:检测非小细胞肺癌(NSCLC)组织中SDF-1、CXCR4、VEGF-C和MMP-9的表达,探讨其及与淋巴结转移的关系。方法:采用免疫组化SP方法检测128例NSCLC组织中SDF-1、CXCR4、VEGF-C和MMP-9的表达,并分析其相关性。结果:SDF-1表达与病理分型(P<0.05)及淋巴结转移(P<0.01)相关,而与分化程度无关,P>0.05。CXCR4表达与病理分型、淋巴结转移和分化程度皆相关,P<0.05。VEGF-C表达与病理分型无关(P>0.05),与淋巴结转移和分化程度相关,P<0.05。MMP-9在肺癌中表达仅与淋巴结转移相关(P<0.05),而与病理分型和分化程度无关,P>0.05。相关性分析显示,SDF-1和CXCR4与VEGF-C高度相关(r值分别为0.704和0.751),提示SDF-1与VEGF-C之间存在相互调控作用。SDF-1与MMP-9相关,r=0.521。结论:SDF-1、CXCR4和VEGF-C协同调节,上调MMP-9表达,促进非小细胞肺癌淋巴结转移。
OBJECTIVE: To detect the expression of SDF-1, CXCR4, VEGF-C and MMP-9 in non-small cell lung cancer (NSCLC) tissues and to investigate their relationship with lymph node metastasis. Methods: The expressions of SDF-1, CXCR4, VEGF-C and MMP-9 in 128 NSCLC tissues were detected by immunohistochemical SP method and the correlation was analyzed. Results: The expression of SDF-1 was correlated with pathological type (P <0.05) and lymph node metastasis (P <0.01), but not with the degree of differentiation (P> 0.05). The expression of CXCR4 correlated with pathological type, lymph node metastasis and differentiation degree (P <0.05). The expression of VEGF-C had no correlation with the pathological type (P> 0.05), but correlated with the degree of lymph node metastasis and differentiation (P <0.05). The expression of MMP-9 in lung cancer was only associated with lymph node metastasis (P <0.05), but not with the pathological type and degree of differentiation (P> 0.05). Correlation analysis showed that SDF-1 and CXCR4 were highly correlated with VEGF-C (r = 0.704 and 0.751, respectively), suggesting a mutual regulation between SDF-1 and VEGF-C. SDF-1 was associated with MMP-9, r = 0.521. Conclusion: SDF-1, CXCR4 and VEGF-C cooperate to regulate MMP-9 expression and promote lymph node metastasis in non-small cell lung cancer.