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目的了解华南地区庚型肝炎病毒（ＨＧＶ）的流行状况及ＨＧＶ不同株和不同基因区的同源性。方法采用逆转录聚合酶链反应技术（ＲＴ－ＰＣＲ）检测来自广东、香港、云南的不同人群血清标本共１９９１份。对其中２０份的５端非编码区（５ＵＴＲ）２３８ｂｐ和３份非结构蛋白５区（ＮＳ５）６２１ｂｐ进行了序列测定和同源性分析。结果一般人群ＨＧＶＲＮＡ阳性率为（０．７３～１．３４）％，献血员（２．５２～２．９０）％，静脉吸毒者１７．８６％，血液透析患者１４．１３％，接受骨髓移植者４１．６７％，在非甲～戊型肝炎病人为２５．３０％，肝细胞癌１４．４８％，乙型肝炎７．２２％，丙型肝炎（８．３３～１６．１３）％。不同株５ＵＴＲ同源性介乎（９０．４０～１００）％；不同株ＮＳ５区核苷酸同源性（９３．３０～９４．００）％，氨基酸为（９７～９９．２）％。结论接受骨髓移植、血液透析、静脉吸毒者，乙型、丙型、非甲～戊型肝炎病人及肝细胞癌患者是ＨＧＶ的高感染人群。不同ＨＧＶ株存在一定的地区性差异；同一地区不同人群的ＨＧＶ株变异不明显；序列中存在高度保守区及较大变异区
Objective To understand the prevalence of hepatitis G virus (HGV) in southern China and the homology of different strains and different gene regions of HGV. Methods Totally 1991 serum samples from different populations in Guangdong, Hong Kong and Yunnan were detected by RT-PCR. Twenty copies of 238 bp of the 5 ’untranslated region (5UTR) and 621bp of the 3 nonstructural protein region 5 (NS5) were sequenced and analyzed for homology. Results The positive rate of HGV RNA in general population was (0.73-1.34)%, blood donors (2.52-2.90%), intravenous drug abusers (17.86%) and hemodialysis patients (14.13% , 41.67%, 25.30% in non-A to E hepatitis, 14.48% in hepatocellular carcinoma, 7.22% in Hepatitis B, and 8.33-16.13% in Hepatitis C virus. The homology of 5 UTR of different strains ranged from 90.40% to 100%. The nucleotide homologies (93.30-94.00%) and amino acids (97-99.2% . Conclusions Patients receiving bone marrow transplantation, hemodialysis, intravenous drug abuse, patients with type B and C, patients with non-hepatitis E and hepatitis E, and patients with hepatocellular carcinoma are highly infected with HGV. Different strains of HGV have some regional differences; HGV strains of different populations in the same area have no significant variation; there are highly conserved regions and large variation regions in the sequences