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目的探讨He-Ne激光血管内照射(ILLLI)对脑梗死患者疗效、血液流变学性质、淋巴细胞增殖、凋亡和染色体的影响。方法脑梗死患者70例随机分成对照组和治疗组,各35例。对照组采用常规药物治疗,治疗组在常规药物治疗的基础上加用He-Ne激光血管内照射治疗,比较两组疗效和治疗前后的血液流变学指标、细胞增殖指数(MI)、细胞周期动力学指数(CKI)、姐妹染色单体交换(SCE)频率及淋巴细胞凋亡频率。结果治疗组有效率88.6%,对照组有效率65.7%,差异有非常显著意义(P<0.05)。治疗组全血黏度(低切,高切)、毛细血管血浆黏度,血沉、血沉方程K值、红细胞聚集指数、红细胞刚性指数较治疗前明显下降,红细胞变形指数较治疗前增加,治疗前后差异有显著意义(P<0.05)。治疗组患者ILLLI后淋巴细胞增殖活性高于对照组(P<0.05),CKI与对照组相比差异无显著意义(P>0.05);两组间SCE频率差异无显著意义(P>0.05);治疗组脑梗死患者的淋巴细胞凋亡率明显高于对照组(P<0.001),也明显高于治疗前(P<0.001)。结论低强度He-Ne激光血管内照射能有效改善血液流变学性质,改善微循环,增加脑血流量,促进神经功能的恢复,显著提高脑梗死患者的疗效,并能提高脑梗死患者的淋巴细胞增殖活性;可诱导发生较高的淋巴细胞凋亡率,对细胞没有致突变性。
Objective To investigate the effects of He-Ne laser intravascular irradiation (ILLLI) on the curative effect, hemorheological properties, lymphocyte proliferation, apoptosis and chromosome in patients with cerebral infarction. Methods 70 patients with cerebral infarction were randomly divided into control group and treatment group, 35 cases each. The control group was treated with conventional drugs. The treatment group was treated with He-Ne laser intravascular irradiation on the basis of conventional drug treatment. The curative effect and the hemorheological indexes before and after treatment were compared between the two groups. The cell proliferation index (MI), cell cycle Kinetic Index (CKI), Sister Chromatid Exchange (SCE) frequency and lymphocyte apoptosis frequency. Results The effective rate was 88.6% in the treatment group and 65.7% in the control group, the difference was significant (P <0.05). The blood viscosity, erythrocyte sedimentation rate, erythrocyte sedimentation rate K value, erythrocyte aggregation index and erythrocyte rigidity index of the treatment group were significantly lower than those before treatment, and the erythrocyte deformability index increased compared with before treatment Significant (P <0.05). The ILLLI lymphocyte proliferative activity in the treatment group was higher than that in the control group (P <0.05). There was no significant difference between the CKI group and the control group (P> 0.05). There was no significant difference in SCE frequency between the two groups (P> 0.05). The apoptosis rate of lymphocytes in the treatment group was significantly higher than that in the control group (P <0.001), and also significantly higher than that before treatment (P <0.001). Conclusion Low intensity He-Ne laser intravascular irradiation can effectively improve the hemorheological properties, improve microcirculation, increase cerebral blood flow, promote the recovery of nerve function, significantly improve the curative effect of patients with cerebral infarction, and can improve the lymphatic Cell proliferation activity; can induce a higher rate of apoptosis of lymphocytes, no mutagenicity on the cells.