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应用已经证明其纯度的纯化病毒株和鸡及地鼠两种免疫血清.用血凝抑制、单扩溶血,株特异性补体结合和中和试验等方法,证明甲型流感病毒H_1N_1末期的变种(Dutch/56)和H_2N_2之间确实存在血清学交叉反应.这种交叉反应用早期H_1N_1变种不能测出.自1957—1966年分离的H_2N_2变种与末期H_1N_1变种都有血清学交叉,但交叉反应滴度逐渐下降.至1967年末分离的最后的H_2H_2变种则完全消失.以单特异性免疫血清或以重组株制备的抗原进行分析,结果表明末期H_1N_1和H_2N_2的血凝素之间存在着抗原关系,而它们的神经氨酸酶则完全不同.对以上的抗原关系,结合H_1N_1末期病毒感染后恢复的病人对H_2N_2有部分保护的流行病学观察.以及H_2N_2病毒起源的重组理论.进行了讨论.
The purified virus strains that have been proved their purity and chicken and hamster serum were used to prove that the end stage variant of H_ (1) N_ (1) of influenza A virus (H_ Dutch / 56) and H_2N_2. This cross-reaction was undetectable with the early H_1N_1 variant.H_2N_2 variants isolated from 1957 to 1966 had serological crosses with the late H_1N_1 variant, but cross-reaction drops Degree gradually decreased.The last H_2H_2 variant separated by the end of 1967 disappeared completely.Analysed by monospecific immune serum or antigen prepared by recombinant strains, the results showed that there was an antigenic relationship between the hemagglutinin of H_1N_1 and H_2N_2, And their neuraminidase is completely different.According to the above antigen relationship, combined with the epidemiological observation of partial protection of H_2N_2 in patients recovered after H_1N_1 end-stage virus infection, and the recombination theory of the origin of H_2N_2 virus are discussed.