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目的探讨尿毒症继发性甲状旁腺功能亢进症(SHPT)患者外周血单个核细胞(PBMC)转录因子GATA3 mRNA和血清γ干扰素(γ-IFN)、白细胞介素4(IL-4)表达水平变化及意义。方法30例尿毒症(CKD5期)SHPT[全段甲状旁腺激素(iPTH)≥300pg/ml]和20例尿毒症非SHPT(iPTH≤150pg/ml)患者,分离PBMCs,RT-PCR半定量法检测GATA3表达水平,ELISA法测γ-IFN、IL-4细胞因子,分析两组间测值的差异及相关性。结果SHPT组GATA3[(1.405±0.493)vs.(0.412±0.093)]和IL-4(65.582±19.043)pg/mlvs.(17.983±4.067)pg/ml]表达水平显著高于非SHPT组,γ-IFN显著低于非SHPT组[(69.842±9.751)pg/mlvs.(79.847±16.991)pg/ml],均P<0.01;GATA3与iPTH及IL-4呈正相关性。结论尿毒症SHPT患者存在免疫功能紊乱,GATA3可能参与其发病机制。
Objective To investigate the expression of GATA3 mRNA, γ-IFN and IL-4 in peripheral blood mononuclear cells (PBMCs) in patients with secondary hyperparathyroidism (SHPT) Horizontal changes and significance. Methods PBMCs were isolated from 30 patients with uremia (CKD stage 5) SHPT [total parathyroid hormone (iPTH) ≥300 pg / ml] and 20 patients without uremia (iPTH≤150 pg / ml). Semiquantitative RT- The expression of GATA3 was detected by ELISA. The cytokines of γ-IFN and IL-4 were measured by ELISA. The difference and correlation between the two groups were analyzed. Results The expression levels of GATA3 [(1.405 ± 0.493) vs. (0.412 ± 0.093)] and IL-4 (65.582 ± 19.043) pg / mlvs. (17.983 ± 4.067) pg / ml in SHPT group were significantly higher than those in non-SHPT group -IFN was significantly lower than that of non-SHPT group [(69.842 ± 9.751) pg / ml vs. (79.847 ± 16.991) pg / ml], both P <0.01; GATA3 was positively correlated with iPTH and IL-4. Conclusions Immune dysfunction exists in patients with uremia SHPT, GATA3 may participate in the pathogenesis.