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目的建立三七药材中人参皂苷Rg1、Rb1和三七皂苷R1的含量测定方法。方法高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,结合固相萃取技术对样品进行预处理。色谱柱:大连伊利特Hypersil氨基柱(200mm×4.0mm,5μm);流动相:乙腈-异丙醇-10mmol·L-1醋酸铵水溶液(冰醋酸调pH5.0)(75∶20∶5);流速:0.6mL·min-1;柱温:室温;ELSD雾化器温度:55℃;氮气流量:2.3L·min-1。结果人参皂苷Rg1、Rb1和三七皂苷R1的线性范围为1.0～10.0μg,加样回收率为95.5%～102.5%,日内精密度≤2%、日间精密度≤4%。结论该方法简便准确,可作为三七的含量测定方法。
Objective To establish a method for the determination of ginsenosides Rg1, Rb1 and notoginsenoside R1 in Radix Notoginseng. Methods High-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) method was used to pretreat samples with solid phase extraction technique. Column: Dalian Hyperlite amino column (200mm × 4.0mm, 5μm); mobile phase: acetonitrile - isopropanol - 10mmol · L-1 ammonium acetate solution (glacial acetic acid adjusted pH5.0) (75:20:5) Flow rate: 0.6 mL·min-1; column temperature: room temperature; ELSD atomizer temperature: 55°C; nitrogen flow rate: 2.3 L·min-1. Results The linear range of ginsenosides Rg1, Rb1 and notoginsenoside R1 was 1.0～10.0μg. The recovery rate of the ginsenosides Rg1, Rb1 and notoginsenoside R1 ranged from 95.5% to 102.5%. The intra-day precision was ≤2% and the inter-day precision was ≤4%. Conclusion This method is simple and accurate and can be used as a method for determination of Panax notoginseng.