论文部分内容阅读
本文对云南首次分离到的Sindbis病毒进行了滤过试验、耐酸耐醚试验、致细胞病变、动物敏感性试验、血凝特性、空斑和毒力等试验研究 ,结果符合披膜病毒科的病毒特性。交叉血抑试验和免疫荧光试验 ,以及空斑减少中和试验进一步证实为甲病毒属的Sindbis病毒。其空斑纯化株的生物学特性也与原株相符 ,纯化株的制备为该株病毒分子生物学研究的准确性和一致性提供了条件。云南Sindbis病毒的首次分离具有重要的流行病学意义 ,其生物学特性研究结果对我省该病的诊断和防治具有重要的指导意义。
In this paper, the first isolation of Sindbis virus in Yunnan were tested, acid and ether resistance test, cytopathic effect, animal susceptibility test, hemagglutination characteristics, plaque and virulence and other experimental studies, the results in line with the Togavirus Division virus characteristic. Cross-seizure and immunofluorescence assays, as well as plaque reduction neutralization, further confirmed the Sindbis virus, an alphavirus. The biological characteristics of the purified plaque were also consistent with those of the original strain. The preparation of the purified strain provided the conditions for the accuracy and consistency of the molecular biology of the strain. The first isolation of Yunnan Sindbis virus has important epidemiological significance, and its biological characteristics of the results of the diagnosis and prevention of the disease in our province has an important guiding significance.