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[目的]对实时荧光定量PCR检测转基因玉米MON863结构特异基因的测量不确定度进行评定。[方法]使用转基因玉米MON863结构特异实时定量PCR方法测定含量为1%的转基因玉米MON863样品(CRM)中结构特异片段的含量,并从扩增反应、数据处理以及微量移液器等不确定度来源,评定测量的不确定度。[结果]研究得到uA=1.7×10-2,uB=9.0×10-4,uC=1.7×10-2,U95=0.036,测量结果为1.08%±0.036。[结论]转基因玉米MON863结构特异实时定量PCR方法检测结果的主要不确定度来自检验过程中的随机效应。
[Objective] The research aimed to evaluate the measurement uncertainty of the gene specific to MON863 in transgenic maize by real-time fluorescence quantitative PCR. [Method] The content of structural specific fragments in transgenic corn MON863 sample (CRM) with content of 1% was determined by the MON863 structured specific real-time quantitative PCR method. The amplification reaction, data processing and uncertainty of micropipette Source, measure the uncertainty of the measurement. [Result] The results showed uA = 1.7 × 10-2, uB = 9.0 × 10-4, uC = 1.7 × 10-2, U95 = 0.036, and the measurement result was 1.08% ± 0.036. [Conclusion] The main uncertainty of MON863 structure-specific real-time PCR method was the random effect of the test.