WT1基因表达对K562、HL-60细胞增殖作用的研究

• 作 者: 秘营昌 王立 卞寿庚 孟庆祥 陈桂彬 罗梅华 沈德诚 王玲 宋鲁燕 崔雯 郭亚林
• 机 构: 中国医学科学院中国协和医科大学血液学研究所血液病医院
• 出 处: 中华血液学杂志
• 发表日期: 1998年12期
• 关键词: 白血病细胞 基因表达 细胞增殖作用 HL-60细胞 WT1基因 寡核苷酸.反义 细胞动力学 逆转录酶 作用机制 细胞系

目的：探讨ＷＴ１基因反义ＲＮＡ对髓系白血病细胞生长增殖和细胞凋亡的影响及作用机制。方法：用ＷＴ１基因反义ＲＮＡ培养Ｋ５６２、ＨＬ６０细胞，用氮蓝四氮唑盐染色、流式细胞仪检测、逆转录聚合酶链反应等方法观察细胞增殖、凋亡、细胞动力学和基因表达情况。结果：ＷＴ１反义ＲＮＡ可以特异性抑制Ｋ５６２细胞增殖，诱导Ｋ５６２、ＨＬ６０细胞凋亡；对ＨＬ６０细胞的增殖无影响，对ＷＴ１、ｂｃｌ２、ｍｄｍ２基因表达无显著影响。结论：ＷＴ１基因与白血病细胞的增殖、凋亡密切相关，对白血病细胞凋亡的影响与ｐ５３、ｂｃｌ２基因无关。

Objective: To investigate the effect of WT1 antisense RNA on the proliferation, proliferation and apoptosis of myeloid leukemia cells and its mechanism. Methods: K562 and HL60 cells were cultured with antisense RNA of WT1 gene. Cell proliferation, apoptosis, and cellular dynamics were observed by nitroblue tetrazolium salt staining, flow cytometry, reverse transcription polymerase chain reaction and other methods. And gene expression. Results: WT1 antisense RNA can specifically inhibit the proliferation of K562 cells, induce the apoptosis of K562 and HL60 cells, have no effect on the proliferation of HL60 cells, and have no significant effect on the expression of WT1, bcl2 and mdm2 genes. Conclusion: WT1 gene is closely related to the proliferation and apoptosis of leukemia cells. The effect of WT1 on the apoptosis of leukemia cells is not related to p53 and bcl-2 genes.