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目的 研究 TNFα在幼兔胎粪吸入后 BALF中的动态变化。方法 :1 )通过气管内灌入胎粪 0 .6 ml/kg和 4ml/kg建立轻、重度幼兔胎粪吸入模型 ;2 )应用放射免疫法检测 BALF中 TNFα含量。结果 :1 )胎粪吸入可引起肺炎症反应 ,表现 BAL F中白细胞数和 PMN增加 ,胎粪吸入后 2 4小时达高峰 ,72小时基本恢复正常 ;2 )胎粪吸入后 BALF中 TNFα含量明显升高 ,1 6～ 2 4小时达高峰 ,72小时接近正常 ,且上述改变於重度胎粪吸入组与轻度胎粪吸入组比较有极显著性差异。与 PMN做相关分析 ,具有明显的相关性。结论 :1 )幼兔胎粪吸入后 ,肺产生明显的炎症反应 ;2 ) TNF。参与胎粪吸入性肺损伤发生发展。
Objective To study the dynamic changes of TNFα in BALF after infant meconium aspiration. Methods: 1) Intrathecal injection of meconium 0.6 kg / kg and 4 ml / kg intratracheal instillation model of mild to severe meconium; 2) the use of radioimmunoassay BALF TNFα levels. Results: 1) Inflammation of the lungs was induced by meconium aspiration. The number of white blood cells and PMN in BALF were increased. The peak of 24 hours after inoculation of meconium reached its peak and returned to normal after 72 hours. 2) TNFα in BALF was significantly increased after meconium aspiration Rise, reach the peak in 1 6 ~ 2 4 hours, close to normal 72 hours, and the above changes in severe meconium aspiration group and mild meconium aspiration group compared with very significant differences. And PMN do related analysis, with obvious relevance. Conclusions: 1) Inflammatory reaction was observed in lungs after meconium in rabbits; 2) TNF. Involved in the development of meconium aspiration lung injury.