论文部分内容阅读
目的观察衰老小鼠血和肺组织抗氧化功能改变及中药杜仲叶的抗氧化性损伤作用。方法用Ｄ－半乳糖建立小鼠代谢紊乱实验性衰老模型，给予不同剂量的杜仲叶水提取物，观察其对小鼠肺和红细胞中超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）及肺和血浆中丙二醛（ＭＤＡ）含量的影响。结果空白对照组的肺ＳＯＤ（ｋＵ／ｇ）、血ＳＯＤ（ｋＵ／ｇ）、肺ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、血ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、肺ＭＤＡ（μｍｏｌ／Ｌ）、血浆ＭＤＡ（μｍｏｌ／Ｌ）分别为：１．５４±０．１５、１．５８±０．０９、６８．８６±９．２１、４７．０９±１０．５０、０．３４±０．０８、４．４９±０．９６；模型组的肺ＳＯＤ（ｋＵ／ｇ）、血ＳＯＤ（ｋＵ／ｇ）、肺ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、血ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、肺ＭＤＡ（μｍｏｌ／Ｌ）、血浆ＭＤＡ（μｍｏｌ／Ｌ）分别为：１．０７±０．３９、１．１６±０．３７、５０．５６±１９．４６、２９．２１±５．８０、０．５７±０．１６、６．１６±０．７４；杜仲叶水提取物７．５ｇ／ｋｇ组的肺ＳＯＤ（ｋＵ／ｇ）、血ＳＯＤ（ｋＵ／ｇ）、肺ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、血ＧＳＨ－Ｐｘ（ｋＵ／ｇ）、肺ＭＤＡ（μｍｏｌ／?
Objective To observe the changes of anti-oxidation function in blood and lung tissues of aging mice and the anti-oxidative damage effect of Eucommia ulmoides Oliv. Leaves. Methods D-galactose was used to establish an experimental aging model of metabolic disorders in mice. Different doses of Eucommia ulmoides aqueous extract were given to observe the superoxide dismutase (SOD) and glutathione peroxide in mouse lung and red blood cells. Effects of enzyme (GSH-Px) and malondialdehyde (MDA) content in lung and plasma. Results Lung SOD (kU/g), blood SOD (kU/g), lung GSH-Px (kU/g), blood GSH-Px (kU/g), lung MDA (μmol/L), plasma in the control group MDA (μmol/L) was 1.54±0.15, 1.58±0.09, 68.86±9.21, 47.09±10.50, 0.34±0.08, 4 respectively. .49±0.96; Model group lung SOD (kU/g), blood SOD (kU/g), lung GSH-Px (kU/g), blood GSH-Px (kU/g), lung MDA (μmol /L), plasma MDA (μmol/L) were: 1.07±0.39, 1.16±0.37, 50.56±19.46, 29.21±5.80, 0.57± 0.16, 6.16±0.74; lung SOD (kU/g), blood SOD (kU/g), lung G of Eucommia ulmoides leaf water extract 7.5 g/kg group H-Px (kU / g), the blood GSH-Px (kU / g), lung MDA (μmol /?