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多聚酶链反应(PCR)为基因组DNA或mRNA的表达突变或序列改变提供了一种快速简便的方法。为研究鼠体细胞在cAMP依赖的蛋白激酶中亚单位的调节,作者建立了特异性扩增mRNA序列和对所扩增DNA进行定序的简易方法。鼠淋巴瘤细胞(S49)mRNA以进一步分离:
Polymerase chain reaction (PCR) provides a quick and easy method for the expression of mutations or sequence changes in genomic DNA or mRNA. To investigate the regulation of murine somatic cells in subunits of cAMP-dependent protein kinases, the authors established a simple method to specifically amplify the mRNA sequence and to sequence the amplified DNA. Murine lymphoma cells (S49) mRNA for further isolation: