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目的:建立一套发根农杆菌高频转化菘蓝植物的有效方法。方法:采用ATCC15834和RI1601两种发根农杆菌并通过超声波处理对草本植物菘蓝的下胚轴、子叶、带子叶下胚轴进行遗传转化,比较了共培养与否对毛状根诱导的影响,并进行了不同抗生素浓度的抑菌效果筛选和毛状根增殖培养基的筛选。结果:浸染后的外植体在不经过共培养阶段的情况下,诱导后的外植体成活率高,毛状根发根时间早,发根率高;经超声波处理后的外植体其毛状根诱导率比未处理高一倍多;同时,筛选出抗生素浓度为400 mg/L时为毛状根生长的最佳抑菌浓度;毛状根在液体培养基中的增殖速度是固体培养基的2～3倍,是普通根的37倍。结论:利用新的方法成功建立了菘蓝的毛状根诱导体系。
Objective: To establish a set of effective methods of Agrobacterium rhizogenes transplanting highland blue plants. Methods: Two strains of A. rhizogenes ATCC15834 and RI1601 were used to transform the hypocotyls, cotyledons and cotyledons hypocotyls of herbaceous plant Bluegrass by ultrasonic treatment. The effects of co-culture on hairy root induction were compared , And antibacterial concentration of antibacterial effect screening and hairy root proliferation medium screening. Results: The explants after inoculation did not undergo co-culture, the survival rate of explants after induction was high, hairy roots with hairy roots were earlier and the rate of hair roots was higher. After ultrasonic treatment, the explants Hairy root induction rate than untreated more than twice; at the same time, screening antibacterial concentration of 400 mg / L for hairy root growth optimum inhibitory concentration; hairy roots in liquid medium proliferation rate is solid Medium 2 to 3 times, is 37 times the ordinary root. Conclusion: The hairy root induction system of Isatis indigotica was successfully established by the new method.