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目的对山东“10·21”辐射事故中2例严重受照射者进行淋巴细胞微核(MN)检测,并估算受照射剂量。方法用胞浆分裂阻滞微核(CBMN)法对2例患者(A和B)的外周血和骨髓样本分别进行MN检测。结果2例患者的外周血培养均未见双核淋巴细胞。患者A的骨髓培养所获双核细胞极少,依据双核淋巴细胞多少粗估剂量>20Gy。患者B的骨髓MN率为2.42个/细胞,剂量估计为8.7(8.0～9.4)Gy,与用染色体畸变分析、物理方法及ESR法所估算剂量接近,与临床表现基本一致。结论MN法简便快速,结果准确,是除染色体畸变分析之外又一种可靠的生物剂量计。
Objective To detect lymphocyte micronuclei (MN) in 2 severe irradiated persons in “10 · 21” radiation accident in Shandong Province and estimate the irradiation dose. Methods Peripheral blood and bone marrow samples from two patients (A and B) were tested for MN by cytoplasmic cleavage-arrest micronuclei (CBMN) assay. Results No peripheral blood mononuclear cells were found in 2 patients. Bone marrow cells obtained in patient A rare bipolar cells, based on the number of binucleated lymphocytes roughly estimated dose> 20Gy. Patient B has a bone marrow MN of 2.42 cells / cell and an estimated dose of 8.7 (8.0-9.4) Gy, which is similar to that estimated by chromosomal aberration analysis, physical method and ESR method, and is consistent with clinical manifestations. Conclusion The MN method is simple, rapid and accurate. It is another reliable biological dosimeter in addition to chromosomal aberration analysis.