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目的观察不同pH值的碱性离子水(alkaline ionized water,AIW)对高脂大鼠的抗氧化作用,探讨其可能的机制。方法选用Wistar雄性大鼠60只,建立大鼠高脂血症模型,给予不同pH值AIW。将大鼠随机分为空白对照组、模型对照组(高脂)、AIW pH 8.5组、AIW pH 9.5组、AIW pH丙二醛10.5组。大鼠饲养8周结束实验,检测血清中TG、TC、HDL、LDL、MDA、SOD及肝脏的MDA含量和SOD活性。用SPSS 11.5软件对结果进行比对分析。结果 pH 8.5和pH 9.5组大鼠血清TC低于模型对照组,差异具有统计学意义(P<0.05),HDL和LDL均明显高于模型对照组,差异均有统计学意义(P<0.05),模型对照组大鼠血清和肝组织SOD活性降低,而MDA含量升高,与空白对照组比较,差异有统计学意义(P<0.01);各AIW组大鼠血清和肝脏组织MDA含量降低,SOD活性升高,与模型对照组比较,差异有统计学意义(P<0.01)。结论 AIW可以提高机体清除氧自由基的能力,具有一定的抗氧化作用。
Objective To observe the antioxidant effects of alkaline ionized water (AIW) with different pH values on hyperlipidemic rats and to explore its possible mechanism. Methods Sixty male Wistar rats were used to establish the model of hyperlipidemia in rats. The rats were given AIW with different pH values. The rats were randomly divided into blank control group, model control group (high fat), AIW pH 8.5 group, AIW pH 9.5 group, AIW pH 10.5 malondialdehyde group. The rats were fed for 8 weeks to finish the experiment. The contents of TG, TC, HDL, LDL, MDA, SOD in the serum and MDA content and SOD activity in the liver were measured. The results were compared using SPSS 11.5 software. Results Compared with model control group, serum TC in pH 8.5 and pH 9.5 groups was significantly lower than that in model control group (P <0.05), HDL and LDL were significantly higher than those in model control group (P <0.05) (P <0.01). The content of MDA in serum and liver tissue of model control group decreased, while the content of MDA increased, compared with the control group, the difference was statistically significant (P <0.01) SOD activity increased compared with the model control group, the difference was statistically significant (P <0.01). Conclusion AIW can improve the ability of the body to scavenge oxygen free radicals and have certain antioxidative effect.