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目的研究胃癌干细胞亚群的筛选方法,探讨建立胃癌干细胞稳定亚群的可行性。方法利用流式细胞仪从MKN45、MKN25、SGC7901细胞株和人胃癌组织中分选出不同CD44和CD133表型的亚群,比较不同亚群和无血清培养基培养的单克隆细胞球细胞在裸鼠移植瘤模型中的成瘤能力。结果不同细胞的CD44+和CD133+亚群在低数量级时几乎不具有裸鼠皮下成瘤能力,在高数量级接种时成瘤能力与母系细胞差异无统计学意义;单克隆细胞球细胞在各数量级下的成瘤能力、瘤体积和生瘤速度均显著高于母系细胞。结论与CD44和CD133等细胞表面标志物筛选法相比,单克隆细胞球细胞可更好的作为胃癌干细胞研究的细胞学基础。
Objective To study the screening method of gastric cancer stem cell subsets and to explore the feasibility of establishing stable subsets of gastric cancer stem cells. Methods The subsets of different CD44 and CD133 phenotypes were sorted out from MKN45, MKN25, SGC7901 cell lines and human gastric cancer cell lines by flow cytometry. The cell population of monoclonal cells cultured in different subgroups and serum-free medium was compared between naked Tumorigenicity in murine xenograft models. Results The subsets of CD44 + and CD133 + in different cells had almost no subcutaneous tumorigenicity in nude mice at low magnitudes. There was no significant difference in the ability of tumorigenesis between males and neonatal mice when inoculated at high order of magnitude. Tumorigenicity, tumor volume and tumor growth rate were significantly higher than maternal cells. Conclusion Compared with the cell surface marker screening methods such as CD44 and CD133, monoclonal cell globosomes may be better cytological basis for gastric cancer stem cell research.