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目的 研究细胞间粘附因子 - 1(ICAM- 1)在乙型肝炎病毒 (HBV)消除及肝癌免疫机制中的意义 ,同时探讨细胞因子对 ICAM- 1的调节作用。方法 利用免疫组化技术对 HBV相关肝病肝组织 ICAM- 1表达进行检测 ;利用细胞酶联免疫技术 ,观察 IFN -γ,IFN -α,TNF对人肝癌细胞H- 740 2 ICAM- 1表达的调节作用。结果 在 CHB中 ,ICAM- 1表达愈强 ,肝内 HBe Ag表达愈少 ;细胞因子对 ICAM- 1表达有增强作用。结论 CHB组织 ICAM- 1表达一定程度有利于 HBV清除 ;I-CAM- 1表达减少是肝癌发生过程中免疫逃逸的重要机制 ;IFN -γ,IFN-α,TNF对 ICAM- 1表达有增强作用 ,通过增强 ICAM- 1表达 ,可能是干扰素治疗乙型肝炎清除 HBV及抗肝癌的重要途径。
Objective To study the significance of intercellular adhesion molecule - 1 (ICAM - 1) in the mechanism of hepatitis B virus (HBV) elimination and hepatocellular carcinoma (HCC) immune response and to investigate the regulation of ICAM - 1 by cytokines. Methods The expression of ICAM-1 in liver tissue of HBV-related liver disease was detected by immunohistochemistry. The expression of ICAM-1 in human hepatocellular carcinoma cell line H-7402 was observed by cell enzyme-linked immunosorbent assay effect. Results In CHB, the stronger the expression of ICAM-1, the less the expression of HBe Ag in the liver. The cytokines enhanced the expression of ICAM-1. Conclusions The expression of ICAM-1 in CHB tissue is beneficial to the clearance of HBV. The decrease of I-CAM-1 expression is an important mechanism of immune escape during the development of hepatocellular carcinoma. The expression of ICAM-1 is enhanced by IFN-γ, IFN- By enhancing the expression of ICAM-1, IFN may be an important way to treat hepatitis B and eliminate HBV and prevent liver cancer.